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CrossRef Phytol stimulates the browning of white adipocytes through the activation of AMP-activated protein kinase (AMPK) in mice fed high-fat diet Fenglin Zhang, Wei Ai, Xiaoquan Hu, Yingying Meng, Cong Yuan, Han Su, Lina Wang, Xiaotong Zhu, Ping Gao, Gang Shu, Qingyan Jiang, Songbo Wang Food & Function.2018
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We drew a standard curve using the gradient dilution of the standard product as a template, added the sample, antibody and HRP aggregation reagent, and put it in a 37C water bath, then washed it five times with a washing solution, we added the chromogenic solution and reacted for 10 min, then detected the absorbance of each hole at a wavelength of 450 nm
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