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25.5 ml of the resulting solution was applied to a 20 ml Source 30S (Amersham Pharmacia Biotech) column equilibrated with 100 ml 0.21 % (w/w) tri-sodium citrate, 0.08 % (w/w) succinic acid, 0.15 % (w/w) di-sodium hydrogen phosphate di-hydrate, 45 % (w/w) ethanol, pH-3.2

Acknowledgements The HCV Genotype Plus RUO assay reagents were kindly provided by Abbott Molecular Inc