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For the in vitro ubiquitination assay, 0.01 g E1, 0.03 g E2, 0.03 g E3, 0.1 g Pelo, and 0.1 g ubiquitin (ThermoFisher Scientific) were incubated in ubiquitination buffer (1 M Tris, pH 7.2, 100 mM ATP, 200 mM MgCl 2 , 40 mM DTT, 20 ) at 30 C

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Designed to manage type 2 diabetes by regulating blood sugar, slowing digestion and reducing appetite, GLP-1s are now taken by millions of people across the world for weight loss, with Australian prescriptions increasing almost 10-fold since 2020 and use in the US more than quadrupling

These findings indicated that the neuroprotective effect of exendin-4 was independent of its blood glucose-lowering effect (51)