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Disinfect the rubber stopper with an alcohol swab and allow it to dry completely
Reconstitute with bacteriostatic water down the inside wall of the vial
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starting immediately after burn and continuing until 24 hours before sacrifice Result:Improved all parameters related to burn wound healing (including reducing edema, inflammatory cell infiltration and necrosis, increasing the number of capillaries, reticulin and collagen fiber formation, preserving hair follicle count, enhancing wound re-epithelization rate, skin breaking strength and relative elongation, and decreasing water content in burned skin)
CDC summarizes that guidance directly in its injection safety recommendations: "Once a multi-dose vial is opened (e.g., needle-punctured) the vial should be dated and discarded within 28 days unless the manufacturer states another date for that opened vial
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