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Clinical translation requires human CSF pharmacokinetic assays to prove BBB bypass, a rigorous dose-escalation Phase I trial to establish a safe human NOAEL for chronic dosing, and an RCT utilizing precise neurocognitive testing (e.g., MoCA) to confirm that murine transcriptomic shifts yield functional neuroprotection in humans

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On the immune side, obesity may cause M2 macrophages ferroptosis due to damage to iron-rich ATMs (MFe hi ) and antioxidant ATMs (Mox), and lead to Treg cells ferroptosis through reductions in NRF2, GPX4, and GCH1 levels

In this context, only the inactive form of vitamin B12 in the human body, cyanocobalamin, is converted to active forms by a single agent, glutathione, to a limited extent

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