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Crosslinking mass spectrometry (XL-MS) The final concentration of 0.5 M DDD-Ube2e2-COP1-c-Jun was incubated with a final concentration of 0.1 mM DSBU crosslinker (Thermo Fisher, A35459) in the wash buffer (20 mM HEPES-NaOH pH 7.4, 200 mM NaCl, 2 mM MgCl 2 , 0.5 mM TCEP) for 40 min at 25 C and quenched with 10 mM Tris-HCl pH 8.0 for 10 min at 25 C

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Ingredients: Purified Water, Ethylhexyl Palmitate, Glycereth-26, Glycerin, Cetearyl Alcohol, Niacinamide, Hydrogenated Poly(C6-14 Olefin), Beeswax, Caprylic/Capric Triglyceride, Polyglyceryl-2 Stearate, Dipropylene Glycol, Isododecane, Glyceryl Stearate, Hydroxyethyl Acrylate/Sodium Acryloyldimethyl Taurate Copolymer, Stearyl Alcohol, Glycol Stearate SE, Sodium Polyacrylate, Hydroxyacetophenone, Stearic Acid, Dimethiconol, Dimethicone/Vinyl Dimethicone Crosspolymer, Hydrogenated Polydecene, Hydrogenated Lecithin, Tranexamic Acid (1,000ppm), Betaine, Glutathione (1,000ppm), Panthenol, Fragrance, Hippophae Rhamnoides Fruit Oil, Butylene Glycol, Adenosine, Sorbitan Isostearate, Caprylyl Glycol, Trideceth-6, 1,2-Hexanediol, Disodium EDTA, Sodium Hyaluronate, Ascorbic Acid