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This process may help progenitor cells of different tissues migrate to a site of injury, thereby facilitating healing [20, 21]

And even if we use sterile water for injection (WFI, water for injection ), we run into another problem: as soon as you pierce the rubber stopper of the vial with a needle and withdraw part of the solution, bacteria from the air can enter the remainder through the same opening

If you increase your dose to get results, you are also increasing your exposure to potentially impure substances

Step 6: Aliquot If Needed If you are preparing multiple doses for an animal study or need to store portions, prepare individual aliquots now using sterile low-binding microcentrifuge tubes