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Where does the available human evidence sit on the study design hierarchy: case report (low grade), retrospective series, or controlled trial (higher grade)

Mitochondrial DNA control region sequence variation in migraine headache and cyclic vomiting syndrome

Typical applications include glutathione S-transferase phase-II conjugation assays, glutathione peroxidase and glutathione reductase coupled assays, GSH/GSSG ratio determination as a redox readout in cultured cells, protein S-glutathionylation studies, and ferroptosis models where glutathione availability governs glutathione peroxidase 4 activity and therefore phospholipid hydroperoxide handling (Yang et al., Cell, 2014)

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The trial did not meet its efficacy endpoint, but tolerability was not the reason the program was discontinued