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doi:10.1210/jc.2004-0431 Bertelsen, J B, and L Hegeds

Sections were deparaffinized in a xylene ethanol series and placed in Tris-EDTA (TE) buffer for antigen retrieval (10 mM Tris, 1 mM EDTA, 0.05% Tween, pH = 9.0) and then blocked in 5% BSA

Retatrutide is an investigational peptide developed by Eli Lilly

A recent randomized controlled trial (RCT) examined the effects of a higher dosage, 3 mg melatonin for 4 weeks, on sleep disturbance in 97 middle-aged patients with primary insomnia [17]