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That damage produces free radicals, which can create even more cell damage

Enzymatic reactions were performed in 96-well plate format (Greiner Bio-One, 655101) with 190 l of reaction mix and 10 l of enzymes diluted in 10 mM sodium phosphate buffer pH 7.0 at the following concentrations: 1.36 g ml 1 (LacAnc100) and 0.81 g ml 1 (LaccID) for ABTS, 1.7 g ml 1 (LacAnc100) and 0.81 g ml 1 (LaccID) for DMP and guaiacol and 6.8 g ml 1 (LacAnc100) and 8.1 g ml 1 (LaccID) for sinapic acid

Inside the cell, metal reductase six-transmembrane epithelial antigen of prostate 3 reduces trivalent iron ions to Fe 2+ , which are then released from the lysosome into the cytoplasm via solute carrier family 11 member 2 for participation in various subsequent physiological and biochemical processes [37, 38]

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