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Biotechniques 38 , 360366 (2005)

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Individuals should be advised to contact their GP or diabetes specialist nurse promptly if they experience: Persistent vomiting or diarrhoea lasting more than 24 hours Inability to maintain adequate fluid intake Signs of dehydration : dizziness, reduced urine output, dark-coloured urine, dry mouth, or excessive thirst Unexplained fatigue or weakness Swelling of the ankles, feet, or face (which may indicate fluid retention or worsening kidney function) Patients with pre-existing kidney disease should be particularly vigilant and may benefit from more frequent contact with their healthcare team during treatment initiation

Rotate injection sites (e.g., abdomen, thighs, upper arms) to minimize local irritation