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Cells were plated on 48-well plates, coated with anti-CD3 (1 g ml 1 ) and anti-CD28 (10 g ml 1 ) antibodies, in RPMI medium supplemented with 10% FCS, penicillin, streptomycin, HEPES, glutamine, pyruvate, nonessential amino acids and -mercaptoethanol diluted with PBS (1:1) in the presence of IL-12 (3 ng ml 1 , Peprotech) (T H 1 conditions)

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For effective results, it should be taken continuously without any interruption as advised by the doctor