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All cryo-EM data were collected on a Titan Krios 300 kV microscope (Thermo Fisher Scientific) equipped with a GIF-Quantum energy filter (Gatan) with the slit set to 20 eV and a Gatan K3 Summit direct electron detection camera (Gatan) at Case Western Reserve University

Data logging thermometers For more detailed monitoring, data loggers record temperatures at set intervals (typically every 15 to 60 minutes) and store the data for later review

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Medical monitoring ensures copper levels stay in safe range