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APX was extracted with 0.1 M KH 2 PO 4 : K 2 HPO 4 (pH 7.0) containing 1 mM EDTA, 1 mM ascorbic acid, 0.5% ( w/v ) polyvinylpolypyrrolidone, and 20% ( w / v ) sorbitol
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It was an active player, releasing chemical messengersincretinsthat acted like advanced scouts, warning the pancreas: Foods on the way, get the insulin ready. By the mid-70s, researchers had narrowed the suspects down to two main hormones: GIP (glucose-dependent insulinotropic polypeptide) GLP-1 (glucagon-like peptide-1), destined to become the star Fast forward to the 1980s
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