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The reverse error would mean injecting more than double

DNA amplifications were performed in a Bio-Rad CFX-96 real-time thermocycler (Bio-Rad, USA), running a standard program (95C for 2 min, 40 cycles at 95C for 10 s, 60C for 10 s and 72C for 30 s, followed by a melting curve analysis to determine amplicon specificity using a temperature range from 65 to 95C with increments of 0.5C)

Limonta P, Moretti RM, Marzagalli M, Fontana F, Raimondi M, Montagnani Marelli M

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