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Estrogen replacement therapy ovarian cancer mortality in a large prospective study of US women

For analysis of mRNA transcripts, cDNA was synthesized from one microgram of DNase Itreated total RNA using iScript cDNA synthesis Kit (Biorad)

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Tissues were then put into 0.15 M sodium cacodylate pH 7.4 containing 2.5% glutaraldehyde and fixed for additional 20 min at 4 C

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