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Although we cannot rule out that the increased production of ROS in 3D culture conditions may not be observed under hypoxia, it should be noted that hypoxia itself induces ROS production in numerous cell types, including tumor cells [39]
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GSTs conjugate 4HNE to GSH, and the corresponding glutathionyl-4-HNE (GS-HNE) is actively transported out of cells via RLIP76, a multifunctional membrane transporter for GSH-conjugated compounds, and/or MRPs (multidrug-resistance proteins
Collectively, the combination of treatment based on PDT, SDT, and CDT promoted the accumulation of highly poisonous 1 O 2 and OH, thus producing a drastically powerful anticancer effect
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