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To determine how this off-target activity may have changed during directed evolution, we transfected wild-type BoNT/X protease at the same concentration

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For morphine if we assume f of 1 and ER of 0.6 then oral bioavailability will be 40%

If uncarboxylated prothrombin is being measured, this "Protein induced by Vitamin K Absence/antagonism (PIVKA-II)" is elevated in vitamin K deficiency

That's not particularly surprising: many users say the regular injections (under the skin) are inconvenient and that the injections are painful with needles that are too large