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Rybelsus is an oral GLP-1 medication FDA-approved for the management of type 2 diabetes, but doctors may prescribe it off-label for weight loss
*Though based in research, personal, and clinical experience, the opinions in this article should not be taken as medical advice
The RTqPCR mixture consisted of 5 L of SYBR Green Master Mix (Yeasen, Shanghai, China), 1 L of upstream primer, 1 L of downstream primer (with a final concentration of 0.2 M for each primer), and 2 L of cDNA, with enzyme-free water added to a final volume of 10 L
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