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This doesnt change the effectiveness of the treatment itself, but it can affect the perceived timeline

Some practitioners use a twice-daily split (e.g., 250 mcg morning, 250 mcg post-workout) for injuries they consider higher grade

When combined with caloric restriction: the same study's diet-plus-injection arm showed 3.2 kg (7 pounds) additional loss versus diet-plus-placebo at 12 weeks, which was statistically significant (p=0.03)

Space this across four to six eating occasions to optimize muscle protein synthesis