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After 48 h, DMEM was replaced with Neurobasal medium (Gibco, 21103049) containing 50 B27 (Gibco, 17504044), 100 glutamine (Invitrogen, 25030081), and 100 penicillin-streptomycin (Thermo, 15140122)
Tobacco Smoke: Cigarette smoke contains 4,000 different chemicals that cause extensive free-radical damage, requiring glutathione for neutralisation
A.MhamdiA.VaultierM
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