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Cells were lysed in 1 mL ChIP lysis buffer (50 mmol/L HEPES-KOH, pH 7.5, 140 mmol/L NaCl, 1 mmol/L EDTA, pH 8.0, 1% Triton X-100, and 0.1% deoxycholate with 0.1 mmol/L PMSF and the EDTA-free protease inhibitor cocktail)

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Action of MT-II on ghrelin-induced feeding in the paraventricular nucleus of the [PMID: 15167567] Laboratory literature summary: Action of MT-II on ghrelin-induced feeding in the paraventricular nucleus of the hypothalamus