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Molecular biology Bacterial expression constructs for wild-type (residues 2-418) and a minimal cysteine (C59A, C125S, C140I, C150V, C242V, C251V, and C269S) and truncated (arr1MC-393) variants of rat arr1 50 , Fab30 51 , and Nb32 11 have been previously reported and functionally verified
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the inability of tertiary amides to donate hydrogen bonds to water (they can only be H-bond acceptors)
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