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Global analysis of the mitochondrial proteome in response to cellular GSH depletion To identify mitochondrial proteins regulated by GSH availability, we used quantitative proteomics and analyzed mitochondria immunopurified from HeLa cells expressing a mitotag (3XHA-OMP25-mCherry) grown in standard media or treated with the glutathione synthesis inhibitor, buthionine sulfoximine (BSO) 13
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Serious complications are uncommon but need quick recognition and response
They are like a series of gears in a clock, which have to have a spring, that is glutathione, to drive the whole process." The body actually produces its own glutathione, which is a peptide, a very small type of protein
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