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This result is consistent with the finding that cAMP signaling serves as the amplification pathway in pancreatic -cells

The cells were incubated at 37 C without 5% CO 2 for 1 h to allow media temperature and pH to reach equilibrium before the first rate measurement

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The progression from one receptor (semaglutide) to two (tirzepatide) to three (retatrutide) follows a clear pattern of increasing efficacy

P1 mice were sacrificed by decapitation, while animals older than 20 days were sacrificed by anesthetic overdose (Pentothal , 0.1 mg/g, i.p., Abbott Australasia Pty