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All participants had a diagnosis of ASD established with DSM-IV-TR or DSM-5 and validated with ADOS-2 and/or ADI-R, while individuals with neurological or psychiatric comorbidities, recent probiotic or oxytocin use, or unstable medications were excluded

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Over recent years two trials with nerve growth factor (NGF) designed to protect forebrain cholinergic neurons (early to moderate stage AD patients) were terminated after phase I [15,16] (NCT00017940, NCT00876863)

But they do not restore normal gastric emptying