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For functional medicine practitioners and researchers interested in this area, understanding what the evidence actually supports versus what remains speculative is the critical starting point

Briefly, a 24-well culture plate was coated with 200 l (9.1 mg/ml)/well Matrigel prepared from Engelbreth-Holm-Swarm mouse tumors (Corning, MA, USA) and allowed to stand for 30 min at 37 C to form a gel layer

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Weekly dosing also means the interval is about one half-life, so drug accumulates and steady state takes four to six weeks