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After 72 h, cells were fixed in 4% PFA for 45 min and then stained with DAPI for 15 min
We first confirmed that cell viability was maintained during the 25-min mechanical test using an MTT assay (Figure S3)
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These distinct RCD subroutines, such as apoptosis, autophagy, pyroptosis, cuproptosis, and ferroptosis, exhibit unique characteristics and displaying significant overlap and crosstalk [71]
They have successfully completed every project we have work on together from modifications to new systems
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