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Cycle length REDEFINE trials ran 68 weeks
GSH or Ni-NTA bead washing was performed four times with cold PBS or washing buffer (20 mM Tris-Cl (pH 8.0), 300 mM NaCl, 20 mM imidazole)
Common research protocols cited in the peptide community use 1 to 2 mg, 3 to 5 times weekly, over 8 to 12-week cycles
Human Fos-related antigen 1 (FOSL1) and Heat Shock 70 kDa Protein 1A (HSPA1A) ELISA kits were purchased prom MyBioSource (San Diego, CA, USA)
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