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Injections are given subcutaneously in the abdomen, thigh or upper arm and are mainly painless
Male mice lacking the entire Mc4r coding region were fertilized with oocytes from female C57BL/6J mice by in vitro fertilization (IVF) to generate heterozygous offspring of both sexes, which were subsequently intercrossed to obtain homozygous knockout mice
This is one of the most effective synergies in the entire protocol
Notably, these findings were concomitant with a decline in ATP levels and the inhibition of oxidative phosphorylation
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