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For electrophysiological recording, cells were seeded onto 12 mm diameter poly-D-lysine coated glass coverslips at a density of in 24-well plates and grown for 48 hours before experiments

Additional modulators, including Acyl-CoA Synthetase Long-Chain Family Member 4 (ACSL4), which promotes PUFA incorporation into membrane phospholipids, and lipoxygenases (LOXs), further influence ferroptosis sensitivity by regulating lipid composition and oxidation dynamics (90, 91)

Our results indicated that neither GHK nor GHK-Cu showed cytotoxic activity at the range of concentrations tested while CuCl 2 and Cu(OAc) 2 showed substantial cytotoxicity to the HaCaT cells at the highest concentration assayed, i.e., at 5800 M, after 30 mins treatment

- Wound Healing: Plays a crucial role in skin integrity and healing processes

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